Friday, March 29, 2013

Imaging methodology reveals nano details not seen before: Understanding nanoparticles at atomic scale in 3-D could improve materials

Mar. 27, 2013 ? A team of scientists from the University of California, Los Angeles (UCLA) and Northwestern University has produced 3-D images and videos of a tiny platinum nanoparticle at atomic resolution that reveal new details of defects in nanomaterials that have not been seen before.

Prior to this work, scientists only had flat, two-dimensional images with which to view the arrangement of atoms. The new imaging methodology developed at UCLA and Northwestern will enable researchers to learn more about a material and its properties by viewing atoms from different angles and seeing how they are arranged in three dimensions.

The study will be published March 27 by the journal Nature.

The authors describe being able to see how the atoms of a platinum nanoparticle -- only 10 namometers in diameter -- are arranged in three dimensions. They also identify how the atoms are arranged around defects in the platinum nanoparticle.

Similar to how CT scans of the brain and body are done in a hospital, the scientists took images of a platinum nanoparticle from many different directions and then pieced the images together using a new method that improved the quality of the images.

This novel method is a combination of three techniques: scanning transmission electron microscopy, equally sloped tomography (EST) and three-dimensional Fourier filtering. Compared to conventional CT, the combined method produces much higher quality 3-D images and allows the direct visualization of atoms inside the platinum nanoparticle in three dimensions.

"Visualizing the arrangement of atoms in materials has played an important role in the evolution of modern science and technology," said Jianwei (John) Miao, who led the work. He is a professor of physics and astronomy at UCLA and a researcher with the California NanoSystems Institute at UCLA.

"Our method allows the 3-D imaging of the local structures in materials at atomic resolution, and it is expected to find application in materials sciences, nanoscience, solid state physics and chemistry," he said.

"It turns out that there are details we can only see when we can look at materials in three dimensions," said co-author Laurence D. Marks, a professor of materials science and engineering at Northwestern's McCormick School of Engineering and Applied Science.

"We have had suspicions for a long time that there was more going on than we could see from the flat images we had," Marks said. "This work is the first demonstration that this is true at the atomic scale."

Nanotechnology expert Pulickel M. Ajayan, the Benjamin M. and Mary Greenwood Anderson Professor of Engineering at Rice University complimented the research.

"This is the first instance where the three-dimensional structure of dislocations in nanoparticles has been directly revealed at atomic resolution," Ajayan said. "The elegant work demonstrates the power of electron tomography and leads to possibilities of directly correlating the structure of nanoparticles to properties, all in full 3-D view."

Defects can influence many properties of materials, and a technique for visualizing these structures at atomic resolution could lead to new insights beneficial to researchers in a wide range of fields.

"Much of what we know about how materials work, whether it is a catalyst in an automobile exhaust system or the display on a smartphone, has come from electron microscope images of how the atoms are arranged," Marks said. "This new imaging method will open up the atomic world of nanoparticles."

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The above story is reprinted from materials provided by Northwestern University. The original article was written by Megan Fellman.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Journal Reference:

  1. Chien-Chun Chen, Chun Zhu, Edward R. White, Chin-Yi Chiu, M. C. Scott, B. C. Regan, Laurence D. Marks, Yu Huang, Jianwei Miao. Three-dimensional imaging of dislocations in a nanoparticle at atomic resolution. Nature, 2013; DOI: 10.1038/nature12009

Note: If no author is given, the source is cited instead.

Disclaimer: Views expressed in this article do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/top_science/~3/KCt2vVQ9aYc/130327144122.htm

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Obama: 'Shame on us if we've forgotten' Newtown (Los Angeles Times)

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GameStick dev unit found at GDC 2013, we go eyes-on

GameStick dev unit found at GDC 2013, we go eyeson

GameStick is here ... er, sort of. The development units are at least here at GDC 2013, quietly hanging out behind a nondescript computer monitor running a few Android games paired with a Nyko wireless controller. That's right, it wasn't running with the proprietary GameStick controller, but a third-party wireless -- we'll go hands-on with the company's internally built controller later this evening, and thusly we didn't want to offer a half-impression with the third-party units available on the show floor. Stay tuned for more, and take a gander at the gallery below for now.

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Source: http://www.engadget.com/2013/03/27/gamestick-dev-unit-eyes-on/

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Thursday, March 28, 2013

Biological transistor enables computing within living cells

Mar. 28, 2013 ? When Charles Babbage prototyped the first computing machine in the 19th century, he imagined using mechanical gears and latches to control information. ENIAC, the first modern computer developed in the 1940s, used vacuum tubes and electricity. Today, computers use transistors made from highly engineered semiconducting materials to carry out their logical operations.

And now a team of Stanford University bioengineers has taken computing beyond mechanics and electronics into the living realm of biology. In a paper to be published March 28 in Science, the team details a biological transistor made from genetic material -- DNA and RNA -- in place of gears or electrons. The team calls its biological transistor the "transcriptor."

"Transcriptors are the key component behind amplifying genetic logic -- akin to the transistor and electronics," said Jerome Bonnet, PhD, a postdoctoral scholar in bioengineering and the paper's lead author.

The creation of the transcriptor allows engineers to compute inside living cells to record, for instance, when cells have been exposed to certain external stimuli or environmental factors, or even to turn on and off cell reproduction as needed.

"Biological computers can be used to study and reprogram living systems, monitor environments and improve cellular therapeutics," said Drew Endy, PhD, assistant professor of bioengineering and the paper's senior author.

The biological computer

In electronics, a transistor controls the flow of electrons along a circuit. Similarly, in biologics, a transcriptor controls the flow of a specific protein, RNA polymerase, as it travels along a strand of DNA.

"We have repurposed a group of natural proteins, called integrases, to realize digital control over the flow of RNA polymerase along DNA, which in turn allowed us to engineer amplifying genetic logic," said Endy.

Using transcriptors, the team has created what are known in electrical engineering as logic gates that can derive true-false answers to virtually any biochemical question that might be posed within a cell.

They refer to their transcriptor-based logic gates as "Boolean Integrase Logic," or "BIL gates" for short.

Transcriptor-based gates alone do not constitute a computer, but they are the third and final component of a biological computer that could operate within individual living cells.

Despite their outward differences, all modern computers, from ENIAC to Apple, share three basic functions: storing, transmitting and performing logical operations on information.

Last year, Endy and his team made news in delivering the other two core components of a fully functional genetic computer. The first was a type of rewritable digital data storage within DNA. They also developed a mechanism for transmitting genetic information from cell to cell, a sort of biological Internet.

It all adds up to creating a computer inside a living cell.

Boole's gold

Digital logic is often referred to as "Boolean logic," after George Boole, the mathematician who proposed the system in 1854. Today, Boolean logic typically takes the form of 1s and 0s within a computer. Answer true, gate open; answer false, gate closed. Open. Closed. On. Off. 1. 0. It's that basic. But it turns out that with just these simple tools and ways of thinking you can accomplish quite a lot.

"AND" and "OR" are just two of the most basic Boolean logic gates. An "AND" gate, for instance, is "true" when both of its inputs are true -- when "a" and "b" are true. An "OR" gate, on the other hand, is true when either or both of its inputs are true.

In a biological setting, the possibilities for logic are as limitless as in electronics, Bonnet explained. "You could test whether a given cell had been exposed to any number of external stimuli -- the presence of glucose and caffeine, for instance. BIL gates would allow you to make that determination and to store that information so you could easily identify those which had been exposed and which had not," he said.

By the same token, you could tell the cell to start or stop reproducing if certain factors were present. And, by coupling BIL gates with the team's biological Internet, it is possible to communicate genetic information from cell to cell to orchestrate the behavior of a group of cells.

"The potential applications are limited only by the imagination of the researcher," said co-author Monica Ortiz, a PhD candidate in bioengineering who demonstrated autonomous cell-to-cell communication of DNA encoding various BIL gates.

Building a transcriptor

To create transcriptors and logic gates, the team used carefully calibrated combinations of enzymes -- the integrases mentioned earlier -- that control the flow of RNA polymerase along strands of DNA. If this were electronics, DNA is the wire and RNA polymerase is the electron.

"The choice of enzymes is important," Bonnet said. "We have been careful to select enzymes that function in bacteria, fungi, plants and animals, so that bio-computers can be engineered within a variety of organisms."

On the technical side, the transcriptor achieves a key similarity between the biological transistor and its semiconducting cousin: signal amplification.

With transcriptors, a very small change in the expression of an integrase can create a very large change in the expression of any two other genes.

To understand the importance of amplification, consider that the transistor was first conceived as a way to replace expensive, inefficient and unreliable vacuum tubes in the amplification of telephone signals for transcontinental phone calls. Electrical signals traveling along wires get weaker the farther they travel, but if you put an amplifier every so often along the way, you can relay the signal across a great distance. The same would hold in biological systems as signals get transmitted among a group of cells.

"It is a concept similar to transistor radios," said Pakpoom Subsoontorn, a PhD candidate in bioengineering and co-author of the study who developed theoretical models to predict the behavior of BIL gates. "Relatively weak radio waves traveling through the air can get amplified into sound."

Public-domain biotechnology

To bring the age of the biological computer to a much speedier reality, Endy and his team have contributed all of BIL gates to the public domain so that others can immediately harness and improve upon the tools.

"Most of biotechnology has not yet been imagined, let alone made true. By freely sharing important basic tools everyone can work better together," Bonnet said.

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The above story is reprinted from materials provided by Stanford University Medical Center. The original article was written by Andrew Myers.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Journal Reference:

  1. Jerome Bonnet, Peter Yin, Monica E. Ortiz, Pakpoom Subsoontorn, and Drew Endy. Amplifying Genetic Logic Gates. Science, 28 March 2013 DOI: 10.1126/science.1232758

Note: If no author is given, the source is cited instead.

Disclaimer: Views expressed in this article do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/top_science/~3/ED1fLVQ-WsM/130328142400.htm

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Source: http://www.rathenauinstituut.com/crucial-search-engine-marketing-strategies-that-really-work/

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Diverse bacteria on fresh fruits, vegetables vary with produce type, farming practices

Mar. 27, 2013 ? Fresh fruit and vegetables carry an abundance of bacteria on their surfaces, not all of which cause disease. In the first study to assess the variety of these non-pathogenic bacteria, scientists report that these surface bacteria vary depending on the type of produce and cultivation practices.

The results are published March 27 in the open access journal PLOS ONE by Jonathan Leff and Noah Fierer at the University of Colorado, Boulder.

The study focused on eleven produce types that are often consumed raw, and found that certain species like spinach, tomatoes and strawberries have similar surface bacteria, with the majority of these microbes belonging to one family. Fruit like apples, peaches and grapes have more variable surface bacterial communities from three or four different groups. The authors also found differences in surface bacteria between produce grown using different farming practices.

The authors suggest several factors that may contribute to the differences they observed, including farm locations, storage temperature or time, and transport conditions. These surface bacteria on produce can impact the rate at which food spoils, and may be the source of typical microbes on kitchen surfaces. Previous studies have shown that although such microbes don't necessarily cause disease, they may still interact with, and perhaps inhibit the growth of disease-causing microbes. The results of this new research suggest that people may be exposed to substantially different bacteria depending on the types of produce they consume.

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The above story is reprinted from materials provided by Public Library of Science.

Note: Materials may be edited for content and length. For further information, please contact the source cited above.


Journal Reference:

  1. Jonathan W. Leff, Noah Fierer. Bacterial Communities Associated with the Surfaces of Fresh Fruits and Vegetables. PLoS ONE, 2013; 8 (3): e59310 DOI: 10.1371/journal.pone.0059310

Note: If no author is given, the source is cited instead.

Disclaimer: This article is not intended to provide medical advice, diagnosis or treatment. Views expressed here do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/kBX0D1wTFq0/130327190542.htm

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Insight: German reliance on Deutsche Bank outweighs scandals

By Edward Taylor and Philipp Halstrick

FRANKFURT (Reuters) - Germany has become so dependent on Deutsche Bank to grease the wheels of its export driven economy that it looks willing to gloss over scandals involving its largest bank.

Deutsche is one of several European banks under investigation by regulators in Europe and the United States for its suspected role in rigging benchmark interest rates. It is cooperating with German authorities in a separate inquiry into alleged tax fraud. Deutsche has denied allegations it misvalued derivatives and mis-sold mortgage-backed securities.

Such an array of inquiries could be expected to damage any bank's reputation. But back-up from business leaders and key members of the bank's supervisory board appear to be helping Deutsche's new co-chief executives Anshu Jain and Juergen Fitschen put the scandals behind them. The two men, with more than 40 years experience at Deutsche between them, took over as co-CEOs on June 1.

This bedrock of support is crucial for Deutsche, especially in a German election year when banks' perceived excesses and misdemeanors could become a campaign issue.

The newest revelations for Deutsche will come in the next few days when the German regulator issues a report on the bank's alleged involvement in the manipulation of Libor, a global interest rate benchmark.

The report will test Germany's commitment to keeping Deutsche strong for the sake of its export led economy. That commitment is a common theme to surface in interviews Reuters has conducted with current and former Deutsche staff, business leaders, sources at the regulator and bank directors.

Several sources familiar with the regulator's report have said it will focus on "organizational flaws" rather than placing blame on Jain or Fitschen, making it less likely the Berlin political establishment will call for them to go.

THE INDUSTRIAL HEARTLAND

A web of support for Deutsche has emerged among German blue-chip and mid-sized companies, which have grown more dependent on the country's largest bank since rivals including IKB, WestLB, LBBW, Commerzbank and Dresdner Bank shut down or slashed international investment banking and lending.

Burkhard Lohr, Chief Financial Officer at K+S Group, a supplier of specialty fertilizers and salt, with activities in Canada, Chile and Brazil said a strong Deutsche was vital. "We need banks with a global network, because our markets are also global," Lohr said.

That view was echoed by Stefan Sturm, Chief Financial Officer of German healthcare group Fresenius SE. "What's crucial is intellectual and financial capital. Particularly in the case of large complex projects which need to be completed seamlessly and in a short period of time," he said.

Thomson Reuters data show how Deutsche's role as lender to German companies has grown since the financial crisis.

In 2008, it ranked only fifth among the biggest lenders to German companies, behind HVB, Dresdner Bank, Royal Bank of Scotland and Commerzbank. Deutsche loaned 4.52 billion euros to German firms, giving it a market share of 7.23 percent.

Four years later, Deutsche Bank is the second-biggest provider of large loans in Germany behind Commerzbank, with a lending volume of 10.82 billion euros, or 15.9 percent, the data show.

The need for a global German bank is even more acute for small and medium sized companies, the backbone of the economy. These small highly specialized manufacturers export goods around the world, but don't have the capacity to maintain multiple relationships with banks to sort out their foreign exchange, interest rate hedging and export finance.

Anshu Jain, who once cultivated trading superstars like Boaz Weinstein and Greg Lippmann, is using his new role to expand support in the "real economy" and in political circles.

Since taking office, the Indian-born banker has met with approximately 50 German chief executives and visited Berlin around 10 times to meet high-ranking politicians.

On one trip, he knocked on the doors of Vorwerk, a maker of vacuum cleaners and kitchen appliances which exports to more than 70 markets from its base in Wuppertal, Germany.

"Anshu Jain came to us well-prepared. He was exceptionally interested in our business," said spokesman Michael Weber. "As an internationally operating company, it is important for us to have as our bank a global partner who is present in many different markets."

WINNING MARKET SHARE

Meanwhile, senior Deutsche staff see a huge opportunity to win market share in an environment which has seen Barclays disrupted by the departure of its CEO and UBS pull out of segments like fixed income.

Crucially for Jain and Fitschen, Deutsche's supervisory board chairman, Paul Achleitner, supports their strategy.

A former Goldman Sachs executive who helped Deutsche make one of its biggest expansions into investment banking in 1998, when he advised it on a deal to buy Bankers Trust, Achleitner is a firm believer in a strong German investment bank.

"What we need as a society is to come to an agreement over what we want. Do we want Germany to be home to a major bank of global importance? There aren't that many companies left in the financial sector capable of competing with U.S. firms," Achleitner said in a written statement in response to questions.

But Deutsche is still paying the price for its more free-wheeling past.

Last week, it was forced to restate its 2012 earnings because of new litigation provisions of 600 million euros related to mortgage-related lawsuits and other regulatory issues including Libor. Seven employees have been suspended or dismissed for suspected involvement in manipulating inter-bank lending rates.

To ensure they retain the support of corporate Germany, Jain and Fitschen need to prove that 'Project Pharos,' a plan to become a more client focused lender really means a change in style. The restructuring efforts, set to be completed by 2015, has already seen about 1,400 jobs axed out of the investment bank, which had 9,094 staff at the end of 2012.

The proprietary trading division, which used the bank's own money to make bets with a notional value of up to $128 billion on mortgage-backed securities, has been shut.

Deutsche has pared back risk taking, reducing the value at risk at its main trading units to 57.1 at the end December, from 95.6 at the end of 2010. A lower number for value-at-risk indicates a reduced likelihood of potential losses.

Internal rivalry once promoted at the bank has been toned down in favor of a greater emphasis on teamwork, insiders say. Sales teams, who once regarded one another as competitors, now coordinate client visits. On the trading floor, the climate is more collegiate.

Traders now get a 'red flag' for breaching rules, including for things previously regarded as trivial - such as failing to attend a compliance course within a five day deadline. Red flags mean lower bonuses and hinder promotion.

The bank has beefed up a ?risk and reputation' committee, which now includes four members of the Group Executive Committee, the bank's 18-member senior management panel. Potentially controversial business is discussed by the head of compliance, the chief risk officer and legal counsel.

Traders are no longer given the kind of leeway they once enjoyed and need to take "MTA" or mandatory time away, surrendering their trading positions to a colleague who can check whether they make sense and conform to risk limits.

Deutsche's problem is that the changes, underway since 2009, take time to filter through to the outside world, insiders say.

"There is a lag between perception and practice," a senior Deutsche Bank executive said.

RESURGENT INVESTMENT BANKERS

Jain's past as a former head of the investment banking division and the expanding influence of that unit, implicated in several of the bank scandals, are part of the reason why 'Project Pharos' has so far struggled to win over critics.

While Barclays signaled a return to its high street roots when it appointed Antony Jenkins, the head of its retail division, to replace former Wall Street trader Bob Diamond as CEO, Deutsche has chosen to promote veterans from the investment bank. Henry Ritchotte, a former chief operating officer (COO) of the investment bank and of the global markets division, is now COO of the entire bank. Michele Faissola, a former global head of rates and commodities, was made head of asset and wealth management.

While trading for years generated the lion's share of profits for Deutsche, it is also the division that is under investigation for alleged interest rate manipulation and the alleged mis-sale of mortgage-backed securities.

Senior Deutsche Bank staff say the reform process is credible.

"Anybody who was involved in anything illegal is no longer with the bank, so it's unfair to keep drawing parallels between now and then," a second senior bank executive said.

But critics says the investment bank's DNA still bears the legacy of Edson Mitchell, the American banker who helped lay the foundations of its global investment banking franchise by introducing a more Anglo-Saxon management style and Wall Street sized paychecks.

"The vast majority at the bank doesn't need a cultural change. It's just the traders," said a Deutsche investment banker specializing in merger and acquisitions. "They have shown over and over again that they care more about themselves than about the bank's reputation."

One of the star bankers Mitchell hired was Jain. Mitchell died in a plane crash in 2000. Today Jain emphasizes greater teamwork between the bank's different divisions.

APPEASING THE GODS

Deutsche Bank has a sometimes uneasy relationship with politicians. The lender angered lawmakers last year when it declined to send Jain to appear before a parliamentary hearing into the Libor scandal. The head of compliance, Stephan Leithner, went instead.

Deutsche also infuriated the German Agriculture Minister and anti-poverty campaigners this year when it decided to lift a self-imposed moratorium on dealing in financial derivatives linked to commodities.

Late last year of bank's Frankfurt headquarters were raided as part of an investigation into tax evasion, money laundering and obstruction of justice over a trading scam involving carbon permits. Fitschen and Stefan Krause, the bank's chief financial officer, are among 25 employees being investigated.

But even if Germany's establishment loses patience with the bank's new leadership, many within Deutsche remain convinced it will be difficult for them to replace Jain and Fitschen.

"Show me the banker who in 2008 did not have some issue. If you want a CEO who is unblemished you need somebody with less than four years experience. You end up with a 25-year-old graduate," a senior Deutsche Banker said, before asking, "Do you have to eliminate investment banking altogether to appease the sacrificial gods."

(Additional reporting by Andreas Kroener and Matthias Sobolewski in Berlin; Editing by Alexander Smith, Carmel Crimmins, Janet McBride)

Source: http://news.yahoo.com/insight-german-reliance-deutsche-bank-outweighs-scandals-111946936--sector.html

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